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The mice had been given free entry to food and water while exercising on the treadmill and housed in a quiet animal room on a 12/12 h mild/darkish cycle with lights on from 6: 00 to 18: 00 at a temperature of 20-22°C and a humidity of 60-65%. The treadmill velocity was set to 2.5 m/min, the running time was 3 s, and the remaining time was 12 s. The treadmill conveyor belt was divided into equal-sized squirrel cages with Plexiglas (30 cm × 30 cm × 40 cm). The treadmill SD mannequin selected in this experiment was first established on the United States Naval College of Aeronautics (WEBB, 1957). This method has been constantly improved, and scientists have step by step acknowledged that the experimental results are comparatively dependable and that this technique decreases the amount of stress imposed on the examined animals (Xu et al., 2010). This method causes a lot much less damage to animals than a traditional water atmosphere or electrical stimulation. If you have any queries concerning in which and how to use buy modafinil online usa, you can speak to us at our own internet site. Finally, the protein focus was decided with a BCA protein detection equipment (Thermo, United States). A Golgi staining kit (PK401, FD Neuro Technologies, Inc., United States) was used according to the manufacturer’s instructions.


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